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Attachment tamer for mail 8.23/22/2023 ![]() ![]() Columns 2–4 represent absorbance at 450 nm, column 5 shows the average mean, column 6 shows standard deviation, and columns 7, 8 contain RSD and RSD%. Controls contain repeated calculation of (Ag +/Apt −), (Ag −/Apt +), biotin directly added into streptavidin-HRP well and PBST-serum+SEB. ![]() Column 1: serial dilution of SEB in the range of 0.002–8 µg/µl coated in each row as shown in the table and controls. This clone presents LOD at the minimum quantity of 5 ng SEB per 100 µl of human serum. Calculation of the limit of detection (LOD) based on HedC2 yielded ssDNA for SEB. Control 1 contains 2 µg SEB without aptamer, control 2 contain 100 nM aptamer without SEB coating. SEA, C and D and human serum were used as counter selection estimation and evaluation. The first row was treated with 100 nM aptamer, the second row with 20, the third row with 4, the fourth with 0.8, the fifth with 0.16 and the sixth was treated with 0.032 nM aptamer. Column SEB c1 was coated with 2 µg SEB, column SEB c2 with 1 µg SEB, column SEB c3 with 0.5 µg SEB, column SEB c4 with 0.25 µg SEB, column SEB c5 with 0.12 µg SEB and column SEB c6 with 0.06 µg SEB. Control 1 (Antigen+-Aptamer−), Control 2 (Antigen-Aptamer+), Control 3: Biotin directly added into streptavidin-HRP well. As shown in the table, clone HedC3 is the most favorable because of its high OD. Column 2, 3 and 4 are obtained from ODs at 450 nm. Binding affinity of different clones in round 11: Column 1 shows different clones and control groups. The results showed that affinity chromatography was an affordable assay with acceptable accuracy to isolate sensitive and selective novel aptamers. In conclusion, aptamers can be considered as useful tools for detecting and evaluating SEB. Results showed that SEB c1 aptamer (2 µg SEB/100 nM aptamer) had favorable specificity to SEB ( k d = 2.3 × 10 −11). Selected aptamers were used to detect SEB in infected serum samples. This study presents a new set of ssDNA aptamer with favorable selectivity to SEB through 12 rounds of SELEX. Among 12 readable sequences, three of them were selected as the most appropriate aptamer because of their affinity and specificity to SEB. The methods used included preparation of a dsDNA library using standard SEB protein as the target analyte, affinity chromatography matrix in microfuge tubes, SELEX procedures to isolate specific ssDNA-aptamer as an affinity ligand, aptamer purification using ethanol precipitation method, affinity binding assay using ELISA, aptamer cloning and specificity test. In this study, we used a new set of ssDNA aptamers against SEB. Aptamers are known as new therapeutic and detection tools which are available in different ssDNA, dsDNA and protein structures. Many diagnostic techniques are based on serological detection and quantification of SEB in different food and clinical samples. Staphylococcal food poisoning (SFP) and respiratory infections mediated by staphylococcal enterotoxin B (SEB) are common clinical manifestations. ![]() From pushing brush to scooping just about anything, this is an essential tool.Staphylococcus aureus are potent human pathogens possessing arsenal of virulence factors. Any time you need a clean separation between the ground and your fill, the Edge Tamer is your tool of choice. Catching an edge or a corner on the lawn while trying to scoop mulch, or filling the bucket with your gravel driveway instead of only snow, are just a few examples of what can happen when you haven’t properly controlled your bucket’s leading edge. For install and use instructions, see the ManualĪ loader bucket is a versatile tool, but not a refined one.Note that 6" Edge Tamers do NOT accept accessories such as the Edge Extender, Trash Tamer, and Hitch Tamer.For replaceable bolt-on edges, see the Bolt-On Tamer.For welded bucket edges (not replaceable bolt-on edges).USPS shipping is per unit ($10/ea for 3"/4", $20/ea for 6").Before ordering, please use our Tamer Guide !. ![]()
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